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相互転座法によるイネの連鎖分析 : II. 染色体5,6,8,9,10,11に対応する連鎖群

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概要 The authors have been engaged in the linkage analysis of rice plants, Oryza sativa L., by the conventional genetic method and reciprocal translocation method. Through the coordination of these two app...roach, ten linkage groups have been assigned to the relevant chromosomes (Table 1). In the previous paper, linkage groups corresponding to the chromosomes 1, 2, 3 and 4 were described. In this paper, the correspondence of the chromosomes 5, 6, 8, 9, 10 and 11 to the linkage groups was presented. Twenty nine kinds o f reciprocal translocation homozygote (Table 2) and twenty one marker genes (Table 3) were used in this study. The following seven genes, described newly by the authors, were contained among these genes (Tables 4-10). dl: A gene for drooping leaf. Midrib of leaf blade and sheath are poorly developed, so cross sections of the basal part of seedling show round shape (Fig. 1). ch: A gene for chlorina. sp: A gene for short panicle. Primary rachis in the lower part of panicles degenerated (Fig. 3). tri: A gene for triangular hull. Tip of lemma recurved over palea (Fig. 2). d_w : A gene for "Waisei-shirasasa" type dwarf (Fig. 4). Twisted leaf and easy threshing. gh_2: A gene for gold hull. Hull and internode take yellowish orange colour by this gene, while only hull is coloured by gh. d_11: A gene for "Shinkane-aikoku" type dwarf. Small and round grain, lax panicle (Fig. 5). The reciprocal translocation homozygotes were crossed with the linkage testers, and F_2 segregations for marker genes and for fertility were examined. The results were shown in Table 11. The recombination values between marker genes and interchanged points were estimated by the maximum likelihood method and the data were shown in Tables 13, 16, 21, 23 and 25. Results of linkage analysis by the conventional genetic method were shown in Tables 12, 14, 15, 17-20, 22 and 24. In these cases, recombination values between marker genes were also estimated by the maximum likelihood method. The conclusions obtained are as follows: 1) The eleventh linkage group, to which bc, dl and ch belong, is corresponded to chromosome 5, and the order of arrangement of these genes and the interchanged points is RT4-5-bc-(RT4-5)-RT2-5-RT3-5-(RT2-5)-dl-RT5-9-ch (Fig. 6). 2) The first linkage group, to which wx, dp_1 , C, ws and Cl belong, is corresponded to chromosome 6, and the order of arrangement is wx-dp_1-RT6-11-C-ws-Cl-RT6-8-RT6-7-(RT6-8) (Fig. 8). 3) The tenth linkage group, which consist of tri, bl_1, d_w and gh_2, is corresponded to chromosome 8, and arrangements of tri-RT7-8a-RT3-8b-bl_1-(RT3-8b)-RT1-8-RT8-10a-d_w-RT7-8b-gh_2 and tri-RT8-10b-bl_1-RT6-8-RT8-10a-d_w-RT3-8a-gh_2-(RT3-8a) are considered (Fig. 9). 4) The eighth linkage group, of which members are la and sp, is corresponded to chromosome 9, and these genes and the interchanged points are arranged in an order of RT9-10-la-RT7-9-RT5-9-sp-(RT5-9) (Fig. 7). 5) The fourth linkage group, of which members are d_6, g and Rc, is corresponded to chromosome 10, and they are arranged in a order of RT6-10-d_6-g-RT8-10a-Rc-RT_1-10-RT8-10b (Fig. 10). 6) The second linkage group, which contains d2, PI, I_g, Ph, Pr and d_11, is corresponded to chromosome 11, and they are arranged in a order of d_2-RT3-11b-Pl-RT10-11_a-lg-RT6-11-Ph (RT6-11)-(RT3-11a)-Pr-RT3-11a-d_II-RT1-11 (Fig. 11).
相互転座をもちいたイネの連鎖分析を行ない,10の染色体に対応する連鎖群を明らかにしたが,ここでは,染色体5,6,8,9,10,11についてのべた. 1. 新たに記載された遺伝子は,たれ葉遺伝子dl,黄緑葉遺伝子ch,三角籾遺伝子tri,黄褐籾遺伝子Sh2,矮性白笹型矮性遺伝子d_w,短穂遺伝子spおよび信金愛国型矮性遣伝子d_11である. 2. bcとdl,dlとchはそれぞれ組換価42.1%,49.2%で連鎖し,dl,chはbcとともに第XI連鎖群を構成する.また,位置関係はbc-dl-chである. 3. 染色体5には第XI連鎖群が対応し,染色体上の遺伝子および転座点の配列順序はRT4-5-bc-(RT4-5)-RT2-5-RT3-5-(RT2-5)-dl-RT5-9-chである. 4. 染色体6にはwx,dp_1,C,ws,Clの属する第I連鎖群が対応し,染色体上の遺伝子および転座点の配列順序はwx-dp_1-RT6-11-C-ws-Cl-(RT6-8)-RT6-7-RT6-8である. 5. bl_1はtriと組換価22.7%で連鎖し,第X連鎖群に属する.また,d_wとgh_2は組換価31.2%で連鎖するが所属連鎖群は不明であつた.しかし,第X連鎖群に対応する染色体8にd_w,gh_2が座乗することが明らかになり,d_w,gh_2はbl_1,triとともに第X連鎖群を構成する.染色体8上の遺伝子および転座点の配列順序はtri-RT7-8a-RT3-8b-bl_1-(RT3-8b)-RT1-8-RT8-10a-d_w-RT7-8b-gh_2,tri-RT8-10b-bl_1-RT6-8-RT8-10a-d_w-RT3-8a-gh_2-(RT3-8a)である. 6. spはlaと組換価20.5%で連鎖し,第VIII連鎖群に属する.この連鎖群には染色体9が対応し,染色体上の遺伝子および転座点の配列順序はRT9-10-la-RT7-9-RT5-9-sp-(RT5-9)である. 7. 染色体10にはd_6,g,Rcの属する第IV連鎖群が対応し,染色体上の遺伝子および転座点の配列順序はRT6-10-d_6-g-RT8-10a-Rc-RT1-10-RT8-10bである. 8. d_11はlg,Ph,Prとそれぞれ組換価37.0%,33.6%,23.0%で連鎖し,したがつて,d_11はd_2,Pl,lg,Ph,Prとともに第II連鎖群を構成する.また,位置関係はd_2-Pl-lg-Ph-Pr-d_11である. 9. 染色体11には第II連鎖群が対応し,染色体上の遣伝子および転座点の配列順序はd_2-RT3-11b-Pl-RT10-11a-lg-RT6-11-Ph-(RT6-11)-(RT3-11a)-Pr-RT3-11a-d_11-RT1-11である. 10. 本研究では染色体番号は西村(1961),連鎖群はNagao and Takahashi(1963)によつたが,いずれは両者は統一すべきである.
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更新日 2020.10.26

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