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Fradiomycin (Neomycin) at above 100 μg/ml drastically stopped the autolysis of Clostridium saccharoperbutylacetonicum, and its other cellular lysis induced by phage HM 2 infection, phage HM 7 infectio...n and a bacteriocin clostocin 0 induction. Similar inhibitory effect of fradiomycin was observed with the cellular lysis induced by penicillin or sodium ion treatment in the co-operation of autolysin. However, phage HM 3-induced lysis was not inhibited by the addition of fradiomycin. In an in vitro-reaction system, fradiomycin also inhibited the action of isolated autolysin, phage HM 2-endolysin, phage HM 7-endolysin and clostocin 0-endolysin upon the formalin-treated organisms or cell wall used as the substrate of C. saccharoperbutylacetonicum, whereas, it did not inhibit the action of isolated phage HM 3-endolysin. Therefore, fradiomytin seemed to be a specific inhibitor against the activities of some lytic enzymes active on C. saccharoperbutylacetonicum.続きを見る
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