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＜紀要論文＞
Antitumoric Potentiality of Enzyme Preparations of Pumpkin Ascorbate Oxidase and Shiitake Mushroom Polyphenol Oxidase

作成者
作成者名
Omura, Hirohisa
大村, 浩久
所属機関
所属機関名
Food Chemistry Institute, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科食糧化学講座
作成者名
Tomita, Yoshifumi
富田, 純史
所属機関
所属機関名
Food Chemistry Institute, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科食糧化学講座
著者識別子
L001137
作成者名
Murakami, Hiroki
村上, 浩紀
所属機関
所属機関名
Food Chemistry Institute, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科食糧化学講座
著者識別子
L001402
作成者名
Nakamura, Yasuhiko
中村, 泰彦
所属機関
所属機関名
Food Chemistry Institute, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科食糧化学講座
本文言語
英語
出版者
Kyushu University
九州大学農学部
発行日
1974-06
収録物名
九州大学大学院農学研究院紀要
巻
18
号
3
開始ページ
191
終了ページ
200
出版タイプ
Version of Record
アクセス権
open access
Crossref DOI
https://doi.org/10.5109/22856
関連DOI
九州大学農学部紀要 || 18(3) || p191-200
Journal of the Faculty of Agriculture, Kyushu University || 18(3) || p191-200
http://www.agr.kyushu-u.ac.jp/
関連URI
九州大学農学部紀要 || 18(3) || p191-200
Journal of the Faculty of Agriculture, Kyushu University || 18(3) || p191-200
http://www.agr.kyushu-u.ac.jp/
関連情報
九州大学農学部紀要 || 18(3) || p191-200
Journal of the Faculty of Agriculture, Kyushu University || 18(3) || p191-200
http://www.agr.kyushu-u.ac.jp/

本文ファイル

pdf p191 pdf 188 KB 472  

詳細

PISSN
0023-6152
NCID
AA00697606
レコードID
22856
査読有無
査読無
主題
Shiitake mushroom
Pumpkin
Antitumoric potentiality
Polyphenol oxidase
Ascorbate oxidase
ISSN
0023-6152
NCID
AA00697606
注記
A crude preparation of ascorbate oxidase was prepared from Japanese pumpkin by treating extract successively with barium acetate, ammonium sulfate, magnesium sulfate and alumina. On the other hand, a purified preparation was obtained by barium acetate treatment, ammonium sulfate fractionation, acetone precipitation, twice chromatography on DEAE cellulose column and gel filtration with sephadex G 100. Both preparations contained antitumoric potentiality against sarcoma-180, even though ascorbate oxidase activity had been decreased after long storage in frozen state or had been completely lost by carboxymethylation of ascorbate oxidase preparation. In addition, antitumoric potentiality was maintained more or less when ascorbate oxidase had been inactivated by heating or splitting off with trypsin. Thus, it was indicated that the antitumoric potentiality of ascorbate oxidase preparation was attributed to the protein itself, but has no intimate relationship with the enzymatic activity. On the other hand, a crude preparation of poIypheno1 oxidase, a similar copper enzyme to ascorbate oxidase, was prepared from shiitake mushroom by treatments with acetone, calcium acetate and ammonium sulfate. Its antitumoric potentiality was also confirmed for sarcoma-180 and MH-134.
登録日 2012.07.20
更新日 2022.11.11

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