Dissolved oxygen consumption by Aerobacter aerogenes was determined by measuring dissolved oxygen change controlling carbon source consumption instead of oxygen consumption by the cells. Results were presented as the oxygen consumed per unit carbon source. The advantage of this method is that measurement was done quickly and precise experimental values were obtained. Oxygen consumption was determined for various carbon sources with the same strain. In these experiments, the values obtained by this method were compared with two other methods. Maltose, pullulan and soluble starch were used as carbon source for isoamylase formation by this strain. Glucose was also used for comparison with the above carbon sources, but it was not a suitable carbon source for isoamylase formation. For the results, coefficient A for oxygen consumption per unit carbon source were calculated for each carbon source and about the same value was obtained for maltose, pullulan and soluble starch respectively, while this value for glucose was half those of the other carbon sources.
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