A method of purification of a deoxyribonuclease I from rice bran was devised. Though it is not the final, the enzyme preparation is purified about 16,000 fold with respect to deoxyribonuclease activity, and the contamination of phosphatase is reduced to a trace. The pH optimum is 5.5. No metal ion is required. Deoxyguanylic acid alone was missing among 5’-mononucleotides identified in the digestion products. The enzyme action appears endonucleolytic.
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