1. In the meristem tissue culture of Calanthe species, a contamination by fungi or bacterium was scarcely observed when an axillary bud as an explant was excised from rapid growing young shoot. Therefore, this study, was carried out with the objects of obtaining aseptic explants in all seasons and increasing survival rate of explants. Firstly, the effect of BA treatment on the shoot formation in axillary buds of pseudobulb was examined. Secondly, after the examination of culture medium condition, micrografting of explant on the protocorm and seedling rootstock was conducted. 2. The treatment of BA promoted shoot formation from the axillary buds of the pseudobulb, and gave the maximum number of shoots by the treatment of 50 ppm concentration of BA. 3. The dilution of inorganic salts of MS medium was effective for an increase of survival rate of explants. 4. A drop falling of l/8 strength of MS liquid medium on the surface of agar medium was effective for the success of the meristem tissue culture. A drop of distilled water was not effective. 5. Micrografting of the meristem tissue was the most effective for the formation of protocorms. More than half number of explants survived and turned to green by all micrograftings. Differentiation in survival rate between each treatments of micrografting was not observed. The optimum survival rate was obtained when the explant was placed on a drop of 1/8 strength of MS liquid medium fell on the apical surface of an incision of the protocorm. 6. Contamination by fungi and/or bacteria in the meristem explants was scarcely observed when explants were excised in young shoots obtained by the treatment of BA. After greening of meristem tissue, the plural number of protocorms were developed from these explants. This fact suggested that rapid mass propagation by proliferating, protocorm would be practicable.