カイコ

Growing ovaries and fertilized eggs in the normal and kidney (ki) mutant which segregated from the reciprocal cross between ki+/ki+and ki+/+ E^ca were used as materials. Protein synthesis and accumulation during oogenesis and changes of yolk protein in the course of embryogenesis were analysed by means of incorporation of ^<14>C-amino acids and electrophoresis. 1. The most active incorporation of ^<14>C-amino acids into ki ovaries was found at the mid pupal stages. Furthermore, it was found that protein contents in the ovaries increased in parallel with the incorporation of radio amino acids and the number of protein bands on gel containing SDS-urea after electrophoresis also as that of the normal ovaries. 2. Quantitative changes of the yolk proteins during the normal embryogenesis were found as gradually decrease. Amount of the soluble proteins at the end of the normal embryogenesis reduced at about 40% of the total soluble protein. On the other hand, changes of the yolk protein contents during ki embryogenesis were found as less amount compared with the normal and about 70% of the total soluble proteins were remained in egg chamber at the end of ki embryogenesis These results suggested that the ovarian (or yolk) proteins in ki ovaries was synthesized normally and deposited into egg chamber, but the yolk protein in embryogenesis of ki eggs made use insufficiently of amino acids source for a formation of embryonic tissues.