Ⅰ) By using agar-agar, 12 aerobic strains have been isolated from bovine rumen of 25 slaughtered cattle. They are five of Bacillus (gram+2 and gram -3), four of Micrococcus (gram+4) and three of Sireptococcus(gram +3).Positive degrees of these isolated strains are 95% in rumen, 85% in reticulum, 65% in omasum, 10% in abomasums. After symbiose test (in vitro) of protozoa and bacteria in bovine rumen, it has been recobnized that 4 strains of Bacillus, and 3 strains of Micrococcus are feeble in their pathogenic-natures. In those strains, the number of bacteria was increased more than that of the control. And I strain of Bacillus which is oval in shape, and 3 strains of Streptococcus which are haemolitic, gelatin-liquefieable, acid-productive from mannitol were decreased more than the control. To study the interrelation between protozoa in rumen and pathogenic strains of organism of intestinal canal, Sal.(pA, pB, typhosa H) and B. coll communis have been used. Sal.(pB, typhosa H) and B. coli communis are seemed to be restrained by protozoa in rumen.
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