焼酎蒸留廃液

The possibility of employing methylated used cooking oil (COME) as extractant to reduce end-product inhibition and to enhance solvent productivity in acetone-butanolethanol (ABE) fermentation was evaluated using oleyl alcohol as the standard butanol extractant. Fermentation was carried out at an initial glucose concentration of 40g/l using TYA medium and shochu distillery waste as fermentation substrate. In the case of TYA medium, butanol production without solvent extractant ceased after 47h cultivation at a concentration of 9.8g/l. The extractant coefficient of oleyl alcohol to butanol was more than three times that of COME to butanol. However, using COME as extractant, total butanol reached 12.6g/l. Using oleyl alcohol, as extractant, on the other hand, total butanol was 11.9g/l. In the case of shochu distillery waste, butanol production without solvent extractant ceased after 50h cultivation at a concentration of 12.5g/l. The extractant coefficient of COME to butanol decreased; that of COME was 0.8 and that of oleyl alcohol was 1.0. Though butanol that extracted using COME as extractant was less than using oleyl alcohol, total butanol reached 16.4g/l and was similar to that using oleyl alcohol as extractant. This fact suggests that COME as extractant is more effective for the reduction of end-product inhibition.