Laboratory of Forest Resources Management, Division of Forest Environment Sciences, Department of Agro-Environment Sciences, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院環境農学部門森林環境科学講座
The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10 mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10^-7 -10^-8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.
Mendeley出力
