Laboratory of Forest Resources Management, Division of Forest Environmental Sciences, Department of Agro-environmental Sciences, Kyushu University
九州大学大学院農学研究院森林資源科学部門森林生態圏管理学講座森林生産制御学研究室
Rhizoctonia praticola was found to produce large amounts of extracellular laccase when grown aerobically on the optimized Lindenberg and Holm medium in fermenter culture with automatic pH control. The laccase from this source was purified to homogeneity by a rapid procedure, using ion-exchange chromatography, affinity chromatography and chromatofocussing. The enzymes isoforms were recovered with a 38- to 115-fold increase in specific activity and a yield for lac1 = 38.93%; lac2 = 14.71% and lac3 = 7.65%. The molecular weight of the purified enzymes proved to be 215, 175 and 68 kDa respectively as determined by size-exclusion HPLC. The isoelectric points were between 6.6 and 7.8, and the carbohydrate content in the purified enzymes was between 6.4-9.7%.
Mendeley出力
