Gut Microbiota of Pheretima heteropoda and Allolobophora japonica Fed under the Same Laboratory Conditions - 九大コレクション

＜紀要論文＞
Gut Microbiota of Pheretima heteropoda and Allolobophora japonica Fed under the Same Laboratory Conditions

作成者	作成者名 NAKAMURA, Kazunori ナカムラ, カズノリ所属機関所属機関名 College of Engineering, Nihon University \| Laboratory of Soils & Environmental Microbiology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental ciences, Kyushu University
作成者	作成者名 SAKAI, Kenji サカイ, ケンジ所属機関所属機関名 Laboratory of Soils & Environmental Microbiology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental ciences, Kyushu University
本文言語	英語
出版者	Faculty of Agriculture, Kyushu University
出版者	九州大学大学院農学研究院
発行日	2018-02-27
収録物名	九州大学大学院農学研究院紀要
巻	63
号	1
開始ページ	71
終了ページ	74
出版タイプ	Version of Record
アクセス権	open access
Crossref DOI	https://doi.org/10.5109/1911179

ファイル	ファイルタイプ	サイズ	閲覧回数	説明
p071	pdf	631 KB	430

PISSN	0023-6152
NCID	AA00697606
レコードID	1911179
主題	Allolobophora japonica
	earthworm
	feeding experiment
	gut microbiota
	Pheretima heteropoda
注記	Our previous study reported that the gut microbiota of Megascolecidae was distinguishable from that of Lumbricidae. In this study, two earthworms, Pheretima heteropoda (Megascolecidae) and Allolobophora japonica (Lumbricidae), were fed under the same in vitro conditions to investigate the effect of feed on gut microbiota. Their gut microbiota was analyzed by denaturing gradient gel electrophoresis of PCR–amplified 16S rRNA gene fragments. Most of the dominant bands were shared between both earthworm species, but some were specific to one species. These banding patterns corresponded to in situ earthworms, i.e. they were directly analyzed after collection from the field. The predominant bacterium found in P. heteropoda gut samples was Bacillus longiquaesitum (AM747042)–related, whereas the predominant bacteria found in A. japonica gut samples were bacterium TG141 (AB308367)–related and Rhizobium sp. BBCT66 (DQ337551)– related. These results indicated that the major bacterial species were not influenced by the surrounding environment, including the food source, even though a large portion of the bacterial population was opportunistic
登録日	2018.03.22
更新日	2023.11.27