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We have been developing a liquid-phase immunoassay using magnetic markers and a supercon-ducting quantum interference device (SQUID). In this method, bound and free markers are magnetically differenti...ated by using the Brownian relaxation of the free markers. Hence, the time consuming washing process that is needed in the conventional optical method for Bound/Free (B/F) separation can be elimi-nated. Using the method, we demonstrated a detection of C-reactive protein (CRP). We obtained good correlation between the detected signal flux and the concentration of CRP. The minimum detectable con-centration was 1 pg/mL. Since the present sensitivity is limited by the blank signal caused by the aggre-gation of free markers, much improvement will be possible if this problem is solved.続きを見る
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paper1(23-1)