An Efficient Protocol of Protocorm–Like Bodies Regeneration from Callus Cultures of Gastrodia elata Blume and the Further Associations with Mycorrhizal Fungi - 九大コレクション

＜紀要論文＞
An Efficient Protocol of Protocorm–Like Bodies Regeneration from Callus Cultures of Gastrodia elata Blume and the Further Associations with Mycorrhizal Fungi

作成者	作成者名 Yeh, Chih-Hsin 所属機関所属機関名 Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
	作成者名 Liao, Fang-Shin 所属機関所属機関名 Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Taoyuan District Agricultural Research and Extension Station, Council of Agriculture
	作成者名 Huang, Kuang-Liang 所属機関所属機関名 Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Department of Horticultural Science, National Chiayi University
	著者識別子 K002580 作成者名 Miyajima, Ikuo 宮島, 郁夫ミヤジマ, イクオ所属機関所属機関名 Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University 九州大学熱帯農学研究センター
	作成者名 Lee, Yung-I 所属機関所属機関名 Institute of Tropical Agriculture, Faculty of Agriculture, Kyushu University \| Biology Department, National Museum of Natural Science \| Department of Life Sciences, National Chung Hsing University
本文言語	英語
出版者	Faculty of Agriculture, Kyushu University
出版者	九州大学大学院農学研究院
発行日	2017-02-24
収録物名	九州大学大学院農学研究院紀要
巻	62
号	1
開始ページ	39
終了ページ	46
出版タイプ	Version of Record
アクセス権	open access
Crossref DOI	https://doi.org/10.5109/1798139

ファイル	ファイルタイプ	サイズ	閲覧回数	説明
p039	pdf	2.48 MB	708

PISSN	0023-6152
NCID	AA00697606
レコードID	1798139
査読有無	査読無
主題	Armillaria
	liquid culture
	mycoheterotrophic orchid
	mycorrhizal fungi
	somatic embryo
ISSN	0023-6152
NCID	AA00697606
注記	In this study, we reported a reliable protocol of PLBs regeneration from embryogenic callus cultures of Gastrodia elata, a fully mycoheterotrophic orchid and an endangered herbal medicinal plant. The continuous immersion cultures with TDZ (0.5 and 1 mg · L^〈–1〉) and BA (10 mg · L^〈–1〉) improved PLBs regeneration frequency and rate. Histological studies indicated that starch grains were abundant within in the cytoplasm of embryogenic callus and developing PLBs. The formation of PLBs from cell aggregates in the periphery region of embryogenic callus was supposed to be the multicellular origin pathway. After inoculated with suitable Armillaria isolates, PLBs enlarged further and resulted in the formation of tubers. The results of phylogenetic analysis and inoculation experiments indicated that the asymbiotic PLBs could establish the appropriate association with Armillaria mellea complex, including A. mellea and A. gallica. Thus, this protocol of PLBs regeneration from callus cultures is beneficial for the multiplication of selected elites, and could be used as an experimental system for the molecular investigations of the mycoheterotrophic orchid – mycorrhizal fungi interactions.
登録日	2017.03.15
更新日	2022.11.11