Nested PCR–based Assay for Diagnosis of Circular Leaf Spot Disease–causing Mycosphaerella nawae and its Application - 九大コレクション | 九州大学附属図書館

＜紀要論文＞
Nested PCR–based Assay for Diagnosis of Circular Leaf Spot Disease–causing Mycosphaerella nawae and its Application

作成者	作成者名 Lee, Seung-Yeol 所属機関所属機関名 Laboratory of Forest Resources Management, Division of Agro–environmental Sciences, Department of Forest Environmental Sciences, Faculty of Agriculture, Kyushu University \| College of Agriculture and Life Sciences, Kyungpook National University
	作成者名 Lim, Yang-Sook 所属機関所属機関名 Laboratory of Forest Resources Management, Division of Agro–environmental Sciences, Department of Forest Environmental Sciences, Faculty of Agriculture, Kyushu University \| Persimmon Experiment Station, Gyeongsangbuk–do Agricultural Research & Extension Services
	作成者名 Jung, Hee-Young 所属機関所属機関名 Laboratory of Forest Resources Management, Division of Agro–environmental Sciences, Department of Forest Environmental Sciences, Faculty of Agriculture, Kyushu University \| College of Agricultural and Life Sciences, Kyungpook National University
	著者識別子 K001681 作成者名 Ohga, Shoji 大賀, 祥治オオガ, ショウジ所属機関所属機関名 Laboratory of Forest Resources Management, Division of Agro–environmental Sciences, Department of Forest Environmental Sciences, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院環境農学部門
本文言語	英語
出版者	Faculty of Agriculture, Kyushu University
出版者	九州大学大学院農学研究院
発行日	2017-02-24
収録物名	九州大学大学院農学研究院紀要
巻	62
号	1
開始ページ	15
終了ページ	21
出版タイプ	Version of Record
アクセス権	open access
Crossref DOI	https://doi.org/10.5109/1798136

本文ファイル

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詳細

PISSN	0023-6152
NCID	AA00697606
レコードID	1798136
査読有無	査読無
主題	Circular leaf spot
	Diagnosis
	Mycosphaerella nawae
	Nested PCR
ISSN	0023-6152
NCID	AA00697606
注記	In this study, we developed a nested PCR detection method based on draft genomic sequences of Mycosphaerella nawae and then applied the method to survey primary symptoms of circular leaf spot (CLS) and the seasonal ascospore scattering period. To develop the assay for detecting the fungus, 11 primers for the initial PCR and 19 primers for the nested PCR were designed (based on the glycoside hydrolase family 3 gene) using draft genomic sequences of M. nawae. Consequently, the primer pairs Mn–F1/ Mn–R1 and Mn–nes–168f/Mn–nes–483r were selected and their specificities examined using regional M. nawae isolates, various healthy persimmon leaves, other fungal pathogens of persimmon, and allied species of M. nawae. Furthermore, the developed nested PCR method was applied for the diagnosis of the initial stage of CLS using both diseased and asymptomatic leaves. Additionally, scattered M. nawae ascospores collected by a spore sampler were surveyed from May to November 2015. Therefore, it is assumed that the nested PCR method can suggest the proper fungicide spraying period for CLS through anticipation of the ascospore quantity, and the early diagnosis of CLS will enable control of its development on persimmon.
登録日	2017.03.15
更新日	2022.11.11