九州大学大学院生物資源環境科学府生命機能科学専攻食料化学工学教育コース食品衛生化学研究室
Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
九州大学大学院生物資源環境科学府生命機能科学専攻食料化学工学教育コース食品衛生化学研究室
Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
九州大学大学院生物資源環境科学府生命機能科学専攻食料化学工学教育コース食品衛生化学研究室
Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学研究室
Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学研究室
Laboratory of Food Hygienic Chemistry, Division of Food Science and Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
Salmonella contamination of spinach leaf during cultivation was investigated. Salmonella Enteritidis expressing green fluorescent protein（S . Enteritidis-GFP）was used in this study. S . Enteritidis-GFP was detected from soil, which had been initially inoculated with the bacterium at more than 2 logCFU/g after 35-d cultivation. The viable S . Enteritidis-GFP counts in soil ranged from 4 to 7 logCFU/g in proportion to the initial inoculum from 2 to 8 logCFU/g after 35-d cultivation. All the 6 spinach plants were negative for S . Enteritidis-GFP by the qualitative test, when spinach had been cultivated in a soil initially contaminated with the bacterium at less than 3.8 logCFU/g. In contrast, all the 6 plants were positive for the bacterium when cultivated in a soil contaminated with more than 6.0 logCFU/g. However, the leaves of all 9 plants were negative for S . Enteritidis-GFP after surface sterilization, suggesting the surface contamination. At 7d after overhead irrigation of water contaminated with S . Enteritidis- GFP at less than 3.1 logCFU/mL, all the spinach plants were negative for the bacterium in 18 separately cultivated spinach plants.
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