In order to investigate the effects of intestinal bacteria on biological characteristics of Riptortus pedestris, these bacteria were isolated and identified from the midgut of field population individuals in 5 outdoor areas and laboratory population. As the result of identification of intestinal bacteria, a total of 8 strains including genus Burkholderia were isolated. Burkholderia sp. was found in 5 all field populations, but there was no Burkholderia sp. in gut of laboratory population. As a result of investigation of growth inhibition of 4 antibiotics (penicillin, ofloxacin, streptomycin, and tetracycline) against isolated 9 intestinal bacteria, ofloxacin was selected as the antibiotic for inhibition of all intestinal bacteria in this study. Selected antibiotic, ofloxacin was treated on soil, the soybean seed, and the host plant and then was provided to R. pedestris as prey. In a result of examination of developmental periods of each larval stage, body length, the number of eggs, the first oviposition time, and hatchability rate of R. pedestris after ofloxacin treatment, it was shown that Burkholderia sp. did not affect the development of the host insect but the first oviposition time was in approximately 60% compared with a control group. Thus, it was thought that the presence of Burkholderia sp. strain affected the number of eggs and the first oviposition time of R. pedestris.