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黒毛和種において給与飼料の違いが，骨格筋形成の抑制調節因子であるマイオスタチンmRNAの発現に如何なる影響を及ぼすかについて検討した．実験区として，出産後3日目で母子分離後，人工哺乳し2カ月齢時より濃厚飼料を多給した濃厚飼料区と，3カ月齢時まで親子放牧後，粗飼料給与のみで飼育した粗飼料区に分けた．それぞれの区における供試牛は2頭とし，5カ月齢時と7カ月齢時においてサンプリングを行い，骨格筋内におけ...るマイオスタチンmRNAの発現の違いについてRT－PCR解析により比較検討した．その結果，両区ともにその発現は確認され，濃厚飼料区で粗飼料区よりも発現が高い傾向がみられた．これらのことより，給与飼料の違いはマイオスタチンmRNA発現に影響を与える可能性が示唆された．
Myostatin, a member of the transforming growth factor(TGF-β)-superfamily, have function as a negative regulator of skeletal muscles growth. We examined the effect of different feeds on myostatin mRNA expression in skeletal muscles of Japanese Black Cattle. The cattle were divided into two groups; one group(two cattle) was mainly fed with concentrate (group-C), another(two cattle) was mainly fed with roughage(group-R). The muscle materials were taken from M.biceps femoris (BF) and M.longissimus thoracis (LT) at 5 months and 7 months of age by needle biopsy method, and stored in liquid nitrogen. After total cellular RNA was extracted from those tissues by ISOGEN, the RT-PCR analysis was carried out. At 5 months of age, in both muscles, the intensity of expression level for myostatin mRNA in group-C was stronger than in group-R. At 7 months of age, in BF, the expression of myostatin mRNA in group-C was stronger than in group-R. However, in LT, the expression made no difference between 2 groups. The present study suggested that the expression of myostatin mRNA in these muscles of calves might be influenced by the feeding system.続きを見る
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