The Efficient Strategy of Plasmid Rescue from Tn5 Mutants Derived from Bradyrhizobium japonicum Is-1, Based on Whole Genome Sequence Information of Strain USDA110 - 九大コレクション

＜紀要論文＞
The Efficient Strategy of Plasmid Rescue from Tn5 Mutants Derived from Bradyrhizobium japonicum Is-1, Based on Whole Genome Sequence Information of Strain USDA110

作成者	作成者名 Tsurumaru, Hirohito 鶴丸, 博人所属機関所属機関名九州大学大学院生物資源環境科学府・独立行政法人製品評価技術基盤機構
	著者識別子 K001595 作成者名 Yamakawa, Takeo 山川, 武夫所属機関所属機関名 Department of Plant Resources Faculty of Agriculture Kyushu University 九州大学大学院農学研究院森林資源科学部門
	作成者名 Tanaka, Masayuki 田中, 將行所属機関所属機関名 Graduate school of Bioresource and Bioenvironmental Sciences, kyushu university・Department of Production ＆ Development, Asahi Food ＆ Healthcare Ltd., 九州大学大学院生物資源環境科学府・アサヒフード＆ヘルスケア生産・開発部
	作成者名 Sakai, Masao 境, 雅夫所属機関所属機関名 Department of Plant Resources Faculty of Agriculture Kyushu University 九州大学大学院農学研究院森林資源科学部門
本文言語	英語
出版者	Faculty of Agriculture, Kyushu University
出版者	九州大学大学院農学研究院
発行日	2008-02-28
収録物名	九州大学大学院農学研究院紀要
巻	53
号	1
開始ページ	27
終了ページ	31
出版タイプ	Version of Record
アクセス権	open access
Crossref DOI	https://doi.org/10.5109/10065
関連情報	Journal of the Faculty of Agriculture, Kyushu University \|\| 53(1) \|\| p27-31
	九州大学大学院農学研究院紀要 \|\| 53(1) \|\| p27-31
	http://www.agr.kyushu-u.ac.jp/index.html

ファイル	ファイルタイプ	サイズ	閲覧回数	説明
p027	pdf	475 KB	402

PISSN	0023-6152
NCID	AA00697606
レコードID	10065
査読有無	査読無
主題	Plant Science
主題	植物学
ISSN	0023-6152
NCID	AA00697606
注記	We previously reported that Tn5 mutants derived from Bradyrhizobium japonicum Is-1 were isolated. In this report, Tn5-encoded kanamycin resistance cassette and its flanking sequence were concurrently cloned into cosmid vector from Tn5 mutants. In general, the genomic southern blot analysis is often performed to select appropriate restriction enzyme before performing the plasmid rescue from cosmid clone. Therefore plasmid rescue experiment consumes the time, especially when more than one Tn5 mutant was obtained. To improve this situation, tentative restriction map of strain Is-1 was constructed based on whole genome sequence information of strain USDA110. Plasmid rescue was performed with restriction enzymes selected according to this map and these enzymes were appropriate for plasmid rescue. This strategy is thought to be effective for saving the time of plasmid rescue in B. japonicum strains other than USDA110.
登録日	2009.04.22
更新日	2022.11.11