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Cap-analysis gene expression (CAGE) : the science of decoding gene transcription

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概要 "The ouput of eukaryotic genomes is much more complex than expected. Genes produce different variants of RNAs from multiple promoters. One of the ultimate targets of biological analysis is to establis...h a relationship between the messenger RNAs that are transcribed from the genome and the genomic regions that control their expression - the promoters - in order to decipher the networks that regulate gene expression and the transcription factors that act as master regulators of transcriptional control."
"Novel technologies have recently appeared that allow deciphering of transcriptional network, based on the identification of the starting site of gene transcription, with the simultaneous measurement of expression level and identification of the promoter elements. These tagging technologies (including cap-analysis gene expression - CAGE - and others) are further boosted from the development of the novel generation of sequencing instruments, which allow transcriptional profiling by sequencing at the cost of microarray experiments.".
"This book is a guide for users of new technologies, as it includes accurately proven protocols, allowing readers to prepare their samples for experiments. Additionally, it provides a guide for the bioinformatics tools that are available for the analysis of the obtained tags, including the design of the software, the sources and web information where they can be downloaded. Finally, the book provides examples of the application of these technologies to identify promoters, annotate genomes, identify new RNAs and reconstruct models of transcriptional control. Although examples mainly regard mammalians, the discussion expands to other groups of eukaryotes, where these approaches are complementing genome sequencing"--P. [4] of cover.
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目次 Cap analysis gene expression (CAGE)
Tagging transcription starting sites with CAGE
Construction of CAGE libraries
Transcriptome and genome characterization using massively parallel paired end tag (PET) sequencing analysis
New era of genome-wide gene expression analysis
Computational tools to analyze CAGE
Extraction and quality control of CAGE tags
Setting CAGE tags in a genomic context
Using CAGE data for quantitative expression
Databases for CAGE visualization and analysis
Computational methods to identify transcription factor binding sites using CAGE information
Transcription regulatory networks analysis using CAGE
Gene-expression ontologies and tag-based expression profiling
Lessons learned from genomic CAGE
Future challenges in CAGE analysis
Comparative genomics and mammalian promoter evolution
The impact of CAGE data on understanding macrophage transcriptional biology.
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アクセス注記 Taylor & Francis eBooks DRM Free Collection トライアル中(2023/3/31まで利用可能) 【Trial】This title is available until March 31, 2023.
本文を見る Taylor & Francis eBooks DRM Free Collection: 2010

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登録日 2023.01.20
更新日 2023.01.20