<電子ブック>
Structural genomics and drug discovery : methods and protocols

責任表示
著者
本文言語
出版者
出版年
出版地
関連情報
概要 Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug disc...overy. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Structural Genomics and Drug Discovery: Methods and Protocols seeks to aid scientists in the further study into structural genomics approach as an efficient initial step toward drug discovery and the methods described will be useful to anyone interested in moving in this direction.--続きを見る
目次 Data Management in the Modern Structural Biology and Biomedical Research Environment
Structural Genomics of Human Proteins
Target Selection for Structural Genomics of Infectious Diseases
Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery
High Throughput Cloning for Biophysical Applications
Expression and Solubility Testing in a High Throughput Environment
Protein Production for Structural Genomics Using E. coli Expression
Eukaryotic Expression Systems for Structural Studies
Automated Cell-free Protein Production Methods for Structural Studies
Parallel Protein Purification
Oxidative Refolding from Inclusion Bodies
High throughput Crystallization Screening
Screening Proteins for NMR Suitability
Salvage or Recovery of Failed Targets by in situ Proteolysis
Salvage of Failed Protein Targets by Reductive Alkylation
Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy
Data Collection for Crystallographic Structure Determination
Structure, Determination, Refinement, and Validation
Virtual High-Throughput Ligand Screening
Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)
Ligand Screening using Enzymatic Assays
Ligand Screening using NMR
Screening Ligands by X-ray Crystallography
Case Study Structural Genomics and Human Protein Kinases.
続きを見る
本文を見る Full text available from Springer Protocols

詳細

レコードID
刊行頻度
主題
SSID
LCCN
NBN
eISBN
登録日 2020.06.27
更新日 2020.06.28