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Meiosis : Volume 2, Cytological Methods

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概要 The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and ...segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 2, Cytological Methods, details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. The methods in this volume are grouped for convenience by general types of organisms: fungi, plants and small animals (mostly invertebrates), and mammals. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Meiosis: Volume 2, Cytological Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.続きを見る
目次 Chromosome Spreading and Immunofluorescence Methods in Saccharomyces cerevisiae
Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading
Assaying Chromosome Pairing by FISH Analysis of Spread Saccharomyces cerevisiae Nuclei
Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe
Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis
Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
Meiotic Cytogenetics in Coprinus cinereus
Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes
Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synaptonemal Complexes From Tomato Microsporocytes.-Cytological Analysis of Meiosis in Caenorhabditis elegans
Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila Spermatoctyes
Methods for Meiotic Chromosome Preparation, Immunofluorescence, and Fluorescence in situ Hybridization in Daphnia pulex
Immunofluorescent Microscopic Study of Meiosis in Zebrafish
Staging of Mouse Seminiferous Tubule Cross-Sections
Isolation and Short-Term Culture of Mouse Spermatocytes for Analysis of Meiosis
Isolation and Analyses of Enriched Populations of Male Mouse Germ Cells by Sedimentation Velocity: The Centrifugal Elutriation
Electron Microscopy of Mammalian Chromosomes
Analyzing Mammalian Female Meiosis
Cytological Analysis of Interference in Mouse Meiosis
Analysis of Telomere Dynamics in Mouse Spermatogenesis
Immunofluorescence Analysis of Human Spermatocytes
Cytological Techniques to Study Human Female Meiotic Prophase
Using RNA FISH to Study Gene Expression During Mammalian Meiosis.
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登録日 2020.06.27
更新日 2020.06.28