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Cyclooxygenases : methods and protocols

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目次 Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns
1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I
Roderick J. Flower
2. Techniques used to study regulation of cyclooxygenase-2 promoter sites
Hiroyasu Inoue and Rieko Nakata
3. Purification of recombinant human COX-1 and COX-2
James K. Gierse
4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2
Nicholas R. Staten and Beverly A. Reitz
5. Expression of cyclooxygenase isoforms in the baculovirus expression system
John C. Hunter, Natalie M. Myres, and Daniel L. Simmons
6. Peroxidase active site activity assay
Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett
7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes
Olivier Boutaud and John A. Oates
8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors
Stefan Laufer and Sabine Luik
9. In vitro cyclooxygenase activity assay in tissue homogenates
Samir S. Ayoub
10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site
William F. Hood
11. In vitro Assays for cyclooxygenase activity and inhibitor characterization
Mark C. Walker and James K. Gierse
Part II. Extraction and measurement of prostanoids and isoprostanes
12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II
Paola Patrignani
13. Prostanoid extraction and measurement
Lorenzo Polenzani and Samir S. Ayoub
14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation
Stefania Tacconelli, Marta L. Capone, and Paola Patrignani
Part III. In vivo models to study involvement of cyclooxygenase products in health and disease
15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III
Derek W. Gilroy, Melanie Stables, and Justine Newson
16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms
Brendan J.R. Whittle
17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats
Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed
18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception
Samir S. Ayoub and Regina M. Botting.
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登録日 2020.06.27
更新日 2020.06.28