<博士論文>
Amphiregulin は LPS 肺傷害マウスにおいて肺胞上皮細胞のアポトーシスを抑制する

作成者
指導教員等
本文言語
学位授与年度
学位授与大学
学位
学位種別
出版タイプ
アクセス権
概要 Background and objective: As a member of the epidermal growth factor family, amphiregulin contributes to the regulation of cell proliferation. Amphiregulin was reported to be upregulated in damaged lung tissues in patients with chronic obstructive pulmonary disease and asthma and in lung epithelial cells in a ventilator-associated lung injury model. In this study, we investigated the effect of amphiregulin on lipopolysaccharide (LPS)-induced acute lung injury in mice. Methods: Acute lung injury was induced by intranasal instillation of LPS in female C57BL/6 mice, and the mice were given intraperitoneal injections of recombinant amphiregulin or phosphate-buffered saline 6 and 0.5 h before and 3 h after LPS instillation. The effect of amphiregulin on apoptosis and apoptotic pathways in a murine lung alveolar type II epithelial cell line (LA-4 cells) were examined using flow cytometry and western blotting, respectively. Results: Recombinant amphiregulin suppressed epithelial cell apoptosis in LPS-induced lung injury in mice. Western blotting revealed that amphiregulin suppressed epithelial cell apoptosis by inhibiting caspase-8 activity. Conclusion: Amphiregulin signaling may be a therapeutic target for LPS-induced lung injury treatment through its prevention of epithelial cell apoptosis.

本文情報を非表示

med2720 pdf 1.45 MB 4 本文
med2720_abstract pdf 398 KB 3 要旨
med2720_review pdf 160 KB 3 審査結果要旨

詳細

レコードID
査読有無
権利関係
関連情報
関連情報
報告番号
学位記番号
授与日(学位/助成/特許)
受理日
部局
登録日 2017.08.21
更新日 2018.01.22