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Productioin of Tetraploid Forms of Acid Citrus Cultivars by Top Grafting of Shoots with Sprouting Axially Buds Treated with Colchicine

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概要 To induce the tetraploid forms of three diploid acid Citrus cultivars (2n=2x=18), 'Yuzu' (C.junos), 'Kizu'(c. kizu), and 'Hanayu'(C. hanaju), sections of one-year-old spring shoots with sprouting axil...lary buds were treated with various concentrations of colcchicine and immediately top-grafted on 30-year-old satsuma mandarin trees at the beginning of April. Characteristics of the plants developed from the top grafted scions werescored five years after the treatments. Tetraploid forms of the cultivars, which were certified by terraploid chromosome number in their seedlings, were derived from the shoot sections treated with 0.05-0.4% colchicine solutions for 12 hours for 'Yuzu', 0.05-0.4% for 6 hours for 'Kizu' and 0.1 and 0.2% for 6 hours for 'Hanayu'. As compared with the original plants, these colchicine-induced tetraploid high leaf shape index (leaf width/ leaf length). The large seeds from open-pollinated diploid cultivars, and their seedlings were tetraploid. These results suggested not only that the tetraploid forms of the three cultivars can be directly used for breding of triploid seedless acid citrus cultivars as seed or pollen parents, but also that the present method is supeeior to the other methods to produce citrus tetraploid dorms directly applicable for tetraproid breeding at two to four years after colchicine treatments.続きを見る

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登録日 2009.04.22
更新日 2017.02.07