Effects of N[6]-benzyladenine(BA) and naphthyl acetic acid(NAA) and of nitrogen concentration in MS medium on in vitro morphogenesis from the rhizomes of Cymbidium sinense cv. Shue Pai Tsoa were investigated. Shoot formation was promoted with higher concentrations of BA irrespective of the presence of NAA. The plants with large number of leaves(>5) were obtained with higher concentrations of both BA and NAA. Reduction of ammonium and potassium nitrate concentrations to 1/4 or 1/2 from the original concentrations enhanced shoot formation. The responsiveness to these treatments was suggested to be common in a wide range of terrestrial Cymbidium species.