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Studies on Potato virus Y Isolates Infecting Potato and Tobacco in Korea

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概要 A survey of Potato virus Y (PVY) aws conducted in farmers' fields in 9 Korean provinces, Gyeonggi-do, Gangwon-do, Gyeongsangnam-do, Gyeongsangbuk-do, Jeollanam-do, Jeollabuk-do, Chungcheongnam-do, Chu...ngcheongbuk-do and Jeju-do from 1998 to 2000. One hundred and fifty nine samples from potato and tobacco were collected and analyzed. Based on ELISA results, three samples PVY-tob, PVY-pot-jj and PVY-pot-chw representing PVY[N] and PVY[O], respectively, were further subjected to biological , serological and molecular analysis. Host range studies showed that PVY-pot-chw is symptomatically distinguishable. However, all the three isoletes failed to infect 10 capsicum varieties systematinally. Isolates PVY-tob and PVY-pot-jj reacted strongly with monoclonal antibody (MAb), anti-PVY[N] while PVY-pot-chw failed to react with the MAb but reacted strongly with anti-PVY[O/C]. Interestingly, both PVY-tob and PVY-pot-jj isolates did not react with anti-PVY[O/C] indicating that isolate PVY-pot-chw is serologically distinguishable from both PVY-tob and PVY-pot-jj. In order to ascertain the toxonomic status of the three isolates, the 3' untranslated terminal regions (3'-UTR) were sequenced. Alignment of the sequences revealed 99% sequence identify between PVY-tob and PVY-pot-jj. both of which had 78% nt sequences identical to PVY-pot-chw. Phylogenetic analysis of the aligned 3'-UTRs clustered PVY-tob and PVY-pot-jj in the PVY[N] subgroup, and PVY-pot-chw in the PVY[O] subgroup.続きを見る

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登録日 2009.04.22
更新日 2017.02.03