<紀要論文>
Isolation of Carp Complement Factor B and Formation of the C3-Convertase

作成者
本文言語
出版者
発行日
収録物名
開始ページ
終了ページ
出版タイプ
アクセス権
概要 Complement factor B (Bf) is a keycomponent that forms a catalytic side of C3-convertase of the alternative activation pathway. However, the functional assay and isolation method has been developed poo...rly for Bf in fish. We describe here a hemolytic assay method for Bf of carp (Cyprinus carpio). The assay derised from prified carp C3 and factor D (Df) is based on the action of Bf which cleaves C3 in the presence of Df. Using this assay method as a probe, carp Bf was isolated from serum, and proved to be a protein comopsed of a single polypeptide with a molecular mass of 93 kDa. This C3-convertase of tje alternative pathway of carp completement was assembled from purified C3, Bf and generation of C3b, the initial biologically active fragment derived from C3, was confirmed, suggesting a well conserved mechanism of the formation of the alternative pathway C3-convertase.続きを見る

本文情報を非表示

p101 pdf 615 KB 192  

詳細

レコードID
査読有無
関連情報
ISSN
NCID
タイプ
登録日 2009.04.22
更新日 2017.02.03