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Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5h after oviposition in a 10mM N-methyl-N-nitrosourea (MNU) solution for 15min. About 80% of thus treated eggs could not hatch. Wh...en observed under a light microscope using unsectioned transparent speciments of unhatched eggs, the embryos exhibited abnormalities such as the absence of innner organs, twisting body axis, incomplete body segments and anomalous segmentation. Those findings confirmed that the immersing method worked well and MNU strongly provokes various formas of somatic impairment in developing embryos.続きを見る
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