<紀要論文>
Freezing Tolerance of Transgenic Tobacco with Increased Content of Unsaturated Fatty Acid by Expressiong the CvFad3 Gene

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概要 Two fatty acid desaturases were independently expressed in tobacco plants and freezing torelance of the transgenic plants was investigated: the two disaturases were CvFAD2, which desaturases microsoma...l C18:1(oleic acid) to C18:2(lonoleic acid), and CvFAD3, which desaturates microsomal C18:2 to C18:3 (linolenic acid). Fatty acud conposition analysis showed that the C18:2 contents in the plants transformed with pBE2113/CvFad2 did not increase compared with that of the wild-type plant. However, an increase in the C18:3 content by 2.4% was observed in the line of No.19 although the increase was not statistically significant. In the plant transformed with pBE2113/CvFad3, the C18:3 content incerased by 6.4% compared with that of the wild-type plant. Measurement of the electolyte leakage of the wild-type plant at all temperatures investigated (from -1 to -4℃). Contrary to the case of the CvFad2, the freezing tolerance of the CvFad3 plant tended to be slightly lower than that of the wild-type at all temperatures tested despite the unsaturation levels of the plant were higher than those of the CvFad2 plants.続きを見る

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登録日 2009.04.22
更新日 2017.01.30