<紀要論文>
Random Amplified Polymorphic DNA(RAPD)解析による黄色ブドウ球菌ゲノムタイピング

作成者
本文言語
出版者
発行日
雑誌名
開始ページ
終了ページ
出版タイプ
アクセス権
概要 To perform the genotyping method of Staphylococcus aureus based on random amplified polymorphic DNA analysis (RAPD), we optimized the several polymerase chain reaction (PCR) conditions such as Mg2+ co...ncentration, annealing temperature and DNA polymerase. In order to assess the discriminatory power of this typing method, 18 epidemiologically unrelated S. aureus strains were examined and the results were compared with the coagulase gone typing method. Among the five primers, RAPD PCR using primer 7 yielding 15 different types was the most discriminative primer. All the strains except two were distinguished each others by the combination of RAPD profiles acquired using the three primers.These data suggest that the discriminatory power of the RAPD genotyping method is great enough to be used as the sole method to type S. aureus and this method is useful as an epidemiological tool.続きを見る

本文情報を非表示

KJ00000075240-00001 pdf 647 KB 114  

詳細

レコードID
selfDOI
査読有無
関連情報
主題
ISSN
NCID
タイプ
時代・年代
登録日 2010.05.17
更新日 2016.09.20