It has been suggested that salivary alpha-amylase (sAMY) reflects sympathetic activity and is a useful stress-related biomarker, but further investigation is needed to confirm this assumption. The purpose of this study was to evaluate whether sAMY activity was associated with speech stress. We measured sAMY activity using a hand-held monitor and self-rating psychological tension before and after a group presentation in front of an audience in a total of 23 university students. The subjects showed a significant decrease in psychological tension following the presentation, but no significant change in sAMY levels was observed. The investigated levels of sAMY were not significantly correlated to those of psychological tension. No obvious gender difference was found in the results. Speech stress in the present study seemed to be insufficient to stimulate sAMY production. Inter-individual differences in the reactivity of sAMY were also considered to affect the results.