Export Mendeley
＜Doctoral Thesis＞
Adipose tissue complement factor B promotes adipocyte maturation
| Creator | |
|---|---|
| Examiner | |
| Language | |
| Academic Year Conferred | |
| Conferring University | |
| Degree | |
| Degree Type | |
| Publication Type | |
| Access Rights | |
| JaLC DOI | |
| Related DOI | |
| Abstract | OBJECTIVES:It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor... B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS:We investigated the expression level of complement system-related genes in adipocytes. To understand the role of CfB in adipocyte, we performed Cfb overexpression in 3T3-L1 preadipocytes and generated adipocyte-specific Cfb transgenic mice. RESULTS:Cfb expression was markedly enhanced in 3T3-L1 adipocytes co-cultured with macrophages following endotoxin stimulation. In Cfb-overexpressing cells, the expression of adipocyte differentiation/maturation-related genes encoding peroxisome proliferator-activated receptor γ (Pparγ), adipocyte Protein 2 and perilipin was significantly enhanced. Cfb transgenic mice showed a marked increase in the expression of genes encoding Pparγ, perilipin, sterol regulatory element-binding protein 1 c, and Cd36 in the subcutaneous adipose tissue. CONCLUSIONS:CfB plays a crucial role in late-phase of adipocyte differentiation and subsequent lipid droplet formation.show more |
Hide fulltext details.
| File | FileType | Size | Views | Description |
|---|---|---|---|---|
dent0765
|
1.86 MB | 753 | 本文 | |
|
dent0765_abstract
|
130 KB | 263 | 要旨 | |
|
dent0765_review
|
213 KB | 193 | 審査結果要旨 |
Details
| Record ID | |
|---|---|
| Peer-Reviewed | |
| Rights | |
| Related PubMed ID | |
| Report Number | |
| Number of Diploma | |
| Granted Date | |
| Date Accepted | |
| Faculty | |
| Created Date | 2018.05.30 |
| Modified Date | 2020.01.31 |