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| Abstract |
This study presents a multimodal characterization of Methylosinus sp. strain C49 (MC49), a Type II methanotroph, to establish a reproducible workflow for methane mitigation and bioplastic polyhydroxya...lkanoate (PHA) production. Cryopreserved cultures exhibited measurable growth by day 10, peaking at OD₆₀₀ 0.357 by day 14. Fluorescence microscopy identified SYBR Green I combined with SlowFade™ Diamond as optimal for cell visualization, while PCR-RFLP confirmed genetic purity across subcultures, storage methods, and environmental conditions, with minor changes after prolonged starvation. This integrated approach provides a reliable framework for methanotroph-based biotechnological applications and underscores critical considerations for culture maintenance, imaging, and molecular validation.show more
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p1368-1377