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Hydroquinone (HQ), a phenolic compound present in industrial processes, cosmetics, and tobacco smoke residues, poses health risks due to its reducing properties and potential to damage DNA and cause c...ancer. Conventional detection methods, such as high-performance liquid chromatography and electrochemical sensors, offer high sensitivity but require complex instrumentation and complex sensor preparation. In this study, we proposed a simple and rapid electrical detection method for HQ based on DNA degradation and dielectrophoretic impedance measurement (DEPIM). Prepared DNA molecules were incubated with HQ to induce degradation and subsequently immobilized on microbeads for DEPIM analysis. The addition of Cu²⁺ ions accelerated the degradation reaction, enabling rapid and sensitive detection. The method demonstrated a clear correlation between the HQ concentration and DEPIM response, with a detection limit of 50 μM and detection time of 60 min. Specificity was confirmed by testing positional isomers, and applicability was validated using artificial urine. The proposed method converts the chemical reaction into an electrical signal through the DEPIM procedure and provides a low-cost, rapid, and straightforward sensing platform for HQ detection, with considerable potential for environmental monitoring and third-hand smoke exposure assessment.続きを見る
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