| 作成者 |
|
|
|
|
|
|
|
| 本文言語 |
|
| 出版者 |
|
| 発行日 |
|
| 収録物名 |
|
| 巻 |
|
| 号 |
|
| 開始ページ |
|
| 終了ページ |
|
| アクセス権 |
|
| 権利関係 |
|
|
|
| 関連DOI |
|
| 関連URI |
|
| 概要 |
We have developed a novel roller conveyer plasma device, which generates gas plasma via atmospheric pressure dielectric barrier discharge (APDBP) on rollers. High voltage and earth electrodes are inco...rporated into the rollers, which are covered with a silicon sheet. Bactericidal activity of the device was measured against Xanthomonas campestris pv. campestris (Xcc), which was spotted onto aluminium plates and subjected to gas plasma treatment generated by APDBD. After treatment, viable cell number of Xcc decreased with a decimal reduction time (D value) of 0.90 min (initial population: 5.0 × 10^8 CFU/ml) and below 0.34 min (initial population: 9.8 × 10^5 CFU/ml). APDBD treatment induced slight morphological changes to Xcc along with significant degradation of lipopolysaccharides (LPS) and degradation/oxidation of genomic DNA to form 8-hydroxy-2'-deoxyguanosine (8-OHdG). Reactive chemical products (hydrogen peroxide, nitrite and nitrate), ultraviolet light (UV-A) and slight temperature elevations were observed during operation of the device. Our results suggest hydrogen peroxide and nitrite generated during operation of the device cause oxidation and degradation of the genomic DNA as well as the degradation of LPS, which are potential mechanisms for inactivation. In addition, the successful inactivation of Xcc on cabbage leaf by the device suggests the method could have practical applications.続きを見る
|
Mendeley出力
