Symmetric or asymmetric protoplast fusions between Solanum sisymbriidorium and othe Solanum species (S. integrifolium and S. toxicarium) were performed to obtain the perfectly soil-borne disease resistant rootstocks for eggplant cultivation. Somatic hybrid was not obtained from symmetric fusion of iodoacetamide-induced inactivation of S. integrifolium and no cell division ability of S. sisymbriifolium on a selective medium in asymmetric fusion, the hybridity of two calli was eluciated by analyzing IDH, PGM and SKDH isozymes. Plant regeneration from the somatic hybrid calli wa also successful, but the regenerated plants did not show the hybidity. The result may be caused by the elimination of S. integrifolium chromosomes during the process of shoot regeneration from calli.
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