Laboratory of Marine Biochemistry ,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Faculty of Agriculture,Kyushu University
Laboratory of Marine Biochemistry,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Graduate School of BIoresource and Bioenvironmental Sciences,Kyushu University
Laboratory of Marine Biochemistry ,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Faculty of Agriculture,Kyushu University
Complement factor B (Bf) is a keycomponent that forms a catalytic side of C3-convertase of the alternative activation pathway. However, the functional assay and isolation method has been developed poorly for Bf in fish. We describe here a hemolytic assay method for Bf of carp (Cyprinus carpio). The assay derised from prified carp C3 and factor D (Df) is based on the action of Bf which cleaves C3 in the presence of Df. Using this assay method as a probe, carp Bf was isolated from serum, and proved to be a protein comopsed of a single polypeptide with a molecular mass of 93 kDa. This C3-convertase of tje alternative pathway of carp completement was assembled from purified C3, Bf and generation of C3b, the initial biologically active fragment derived from C3, was confirmed, suggesting a well conserved mechanism of the formation of the alternative pathway C3-convertase.
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