Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27 - Collections

＜departmental bulletin paper＞
Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27

Creator	Author PID K001621 Creator Name Honjoh, Ken-ichi 本城, 賢一ホンジョウ, ケンイチ Affiliation Affiliation Name Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
	Creator Name Suga, Koushirou 菅, 向志郎スガ, コウシロウ Affiliation Affiliation Name Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
	Creator Name Shinohara, Fuminori 篠原, 史訓シノハラ, フミノリ Affiliation Affiliation Name Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University
	Creator Name Maruyama, Isao 丸山, 功マルヤマ, イサオ Affiliation Affiliation Name Chlorella Industries Co.Ltd.
	Author PID K001577 Creator Name Miyamoto, Takahisa 宮本, 敬久ミヤモト, タカヒサ
	Creator Name Hatano, Shoji 波多野, 昌二ハタノ, ショウジ Affiliation Affiliation Name Graduate School if Healts and Social Welfare Science, Nishikyushu University
	Creator Name Iio, Masayoshi 飯尾, 雅嘉イイオ, マサヨシ
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2003-02-01
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	47
Issue	2
First Page	257
Last Page	266
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/4494
Related DOI	Journal of the Faculty of Agriculture, Kyushu University \|\| 47(2) \|\| p257-266
	九州大学大学院農学研究院紀要 \|\| 47(2) \|\| p257-266
	http://www.agr.kyushu-u.ac.jp/index.html
Related URI	Journal of the Faculty of Agriculture, Kyushu University \|\| 47(2) \|\| p257-266
	九州大学大学院農学研究院紀要 \|\| 47(2) \|\| p257-266
	http://www.agr.kyushu-u.ac.jp/index.html
Relation	Journal of the Faculty of Agriculture, Kyushu University \|\| 47(2) \|\| p257-266
	九州大学大学院農学研究院紀要 \|\| 47(2) \|\| p257-266
	http://www.agr.kyushu-u.ac.jp/index.html

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PISSN	0023-6152
NCID	AA00697606
Record ID	4494
Peer-Reviewed	Unrefereed
ISSN	0023-6152
NCID	AA00697606
Notes	Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of naked protoplasts was confirmed by fluorescence microscopy using fluorescent brightner 28, which stains cell walls. About 88% of C. vulgaris K-73112 cells were covereted into osmotically-labile cells. Futhermore, a method for regeneration of intact cells from the protoplasts was developed. Utilization of 0.5M sucrose as an osmoticum, Fe-EDITA as an iron source, and bacto-agar as a supporting was shown to help regeneration of the cell walls of two strains, C. vulgaris K-73112 and C-27.
Created Date	2009.04.22
Modified Date	2022.11.11

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＜departmental bulletin paper＞ Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27

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＜departmental bulletin paper＞
Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27