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Plants increase sulfate uptake activity under sulfur deficiency (−S). In Arabidopsis, SULTR1;2 is the major highaffinity sulfate transporter induced in epidermis and cortex of roots for mediating sulf...ate uptake under −S. Though it is known that transcript levels of SULTR1;2 increase under −S largely due to the function of 5′-upstream region, contributions of 5′-non-transcribed flanking region and 5′-untranslated region (UTR) to transcriptional and post-transcriptional regulations have not yet been individually verified. To investigate the roles of 5′UTR of SULTR1;2 in −S responses, transcript levels and activities of firefly luciferase (Luc) were analyzed in transgenic plants expressing Luc under the control of the 2,160-bp long 5′-upstream region of SULTR1;2 with (PL2160) or without (PL2160ΔUTR) the 154-bp 5′UTR. Both transgenic plants expressed similar levels of Luc mRNAs that showed significant accumulations under −S relative to +S regardless of presence of the 5′UTR. In contrast, Luc activities were detected only in PL2160 plants, suggesting presence of 5′UTR of SULTR1;2 being necessary for translational initiation while its absence impairing translation of functional Luc protein in PL2160ΔUTR. These results indicate an essential role of the 5′-non-transcribed flanking region of SULTR1;2 at positions −2160 to −155 in −S-responsive transcriptional regulation.続きを見る
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