Nutrient limitation alters the levels of two distinct forms of cyclin–dependent kinase G2, a putative autophagy progression modulator in tobacco BY–2 cells - Collections | Kyushu University Library

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＜departmental bulletin paper＞
Nutrient limitation alters the levels of two distinct forms of cyclin–dependent kinase G2, a putative autophagy progression modulator in tobacco BY–2 cells

Creator	Creator Name NAGAHAMA, Masaki 長濱, 正記ナガハマ, マサキ Affiliation Affiliation Name Laboratory of Plant Nutrition, Division of Soil Science and Plant Nutrition, Department of Plant Resources, Graduate School of Bioenvironmental Sciences, Kyushu University 九州大学大学院農学研究院
	Creator Name ASATSUMA, Satoru 浅妻, 悟アサツマ, サトル Affiliation Affiliation Name Laboratory of Plant Nutrition, Division of Soil Science and Plant Nutrition, Department of Plant Resources, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院 Affiliation Affiliation Name Cell Function Research Team, RIKEN Plant Science Center
	Creator Name SHIMIZU, Masami シミズ, マサミ Affiliation Affiliation Name Laboratory of Horticultural Science, Division of Agrobiological, Sciences, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院
	Author PID K003062 Creator Name MATSUOKA, Ken 松岡, 健マツオカ, ケン Affiliation Affiliation Name Laboratory of Plant Nutrition, Division of Soil Science and Plant Nutrition, Department of Plant Resources, Graduate School of Bioenvironmental Sciences, Kyushu University 九州大学大学院農学研究院 Affiliation Affiliation Name Laboratory of Plant Nutrition, Division of Soil Science and Plant Nutrition, Department of Plant Resources, Faculty of Agriculture, Kyushu University 九州大学大学院農学研究院 Affiliation Affiliation Name Cell Function Research Team, RIKEN Plant Science Center
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2021-03-01
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	66
Issue	1
First Page	1
Last Page	10
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/4363545

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PISSN	0023-6152
NCID	AA00697606
Record ID	4363545
Subject Terms	alternative splicing
	chimeracell cycle
	starvation
	sucrose
Notes	By screening protein kinases that retard autophagy progression in tobacco (Nicotiana tabacum) BY–2 cells, we found that a tobacco cyclin–dependent kinase G2 (NtCDKG2) would be involved in autophagy progression under sucrose–starvation condition. Characterization of its cDNA, and comparison with corresponding mRNA and genome sequences in parental species of tobacco indicated that the NtCDKG2 gene originated from N. tomentosiformis. Comparison of the deduced amino acid sequences of NtCDKG2 cDNA with those of N. tomentosiformis indicated that the protein encoded by the cDNA (NtCDKG2;1) is identical to a shorter variant of the CDKG2 protein. Characterization of NtCDKG2;1 protein using specific antibodies indicated that 73 kDa and 48 kDa forms, designated as NtCDKG2;1p73 and NtCDKG2;1p48, present in tobacco BY–2 cells. NtCDKG2;1p73 decreased under starvation of either sucrose or phosphate whereas NtCDKG2;1p48 decreased under starvation of sucrose and nitrogen. These data suggest that the roles of these two forms differ in tobacco cells.
Funding Information	Funder Name Japan Society for the Promotion of Science 日本学術振興会 Award Number 19380045 Award Title Induction mechanism of intracellular protein degradation under nutrient starvation in plants 植物における細胞内分解系の栄養欠乏による誘導機構
Funding Information	Funder Name Japan Society for the Promotion of Science 日本学術振興会 Award Number 26292194 Award Title Analysis and function enhancement of plant vesicle transport system as the basis of plant biomass production control 植物バイオマス生産制御の基盤となる植物小胞輸送系の解析と機能増強
Created Date	2021.03.03
Modified Date	2022.11.11