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We have developed a liquid phase immunoassay technique using the Brownian relaxation of magnetic markers. In order to achieve this, we used large polymer beads to fix the bound markers. The bound and ...unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation. We used MR (magneto-Resistive) sensor for the signal detection. In order to achieve high sensitivity and low noise of the sensor, we used a modulation technique called set/reset operation. Using this method, magnetic field resolution of the MR sensor was improved by about three times. We also set up a magnetic shield to prevent the influence of an environmental magnetic field. We show that the present system could detect 20 ng of magnetic markers. We also demonstrate the detection of 13.8 amol/ml of biotin with the present liquid-phase immunoassay technique.show more
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