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＜departmental bulletin paper＞
Detection of Penicillin-Binding Proteins in Bacillus Cereus by Using Biotinylated β-Lactams

Creator
Author PID
K001577
Creator Name
Miyamoto, Takahisa
宮本, 敬久
Affiliation
Affiliation Name
Laboratory of Food Hygienic Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University
九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学分野
Creator Name
Sukimoto, Kouji
Affiliation
Affiliation Name
Laboratory of Food Hygienic Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University
Creator Name
Sayed, Md Abu
Affiliation
Affiliation Name
Laboratory of Food Hygienic Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University
Creator Name
Kim, Sam-In
Affiliation
Affiliation Name
Laboratory of Food Hygienic Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University
Author PID
K001621
Creator Name
Honjoh, Ken-ichi
本城, 賢一
Affiliation
Affiliation Name
Laboratory of Food Hygienic Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University
九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学分野
Creator Name
Hatano, Shoji
波多野, 昌二
Affiliation
Affiliation Name
Laboratory of Food Hygienic Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University
九州大学大学院農学研究院生命機能科学部門食料化学工学講座食品衛生化学分野
Language
English
Publisher
Kyushu University
九州大学農学部
Date
2000-02
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
44
Issue
3/4
First Page
299
Last Page
307
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/24332
Related DOI
九州大学農学部紀要 || 44(3/4) || p299-307
Journal of the Faculty of Agriculture, Kyushu University || 44(3/4) || p299-307
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 44(3/4) || p299-307
Journal of the Faculty of Agriculture, Kyushu University || 44(3/4) || p299-307
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 44(3/4) || p299-307
Journal of the Faculty of Agriculture, Kyushu University || 44(3/4) || p299-307
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
24332
Peer-Reviewed
Unrefereed
Subject Terms
Penicillin binding proteins (PBPs)
Biotin-ampicillin conjugate (Bio-PCA)
β-Lactams
ISSN
0023-6152
NCID
AA00697606
Notes
A new method for detection of penicillin binding proteins from bacterial membranes has been developed in this study. The method, that employed biotin-ampicillin conjugate (Bio-PCA), is very rapid and can be a significant alternative of hazardous and time consumimng conventional radiometric method for detection of PBPs in cell membranes. PRPs from B. cereus were examined by this technique. In vegetative and sporulating cells, 8 major PBPs were detected. Comparing with standard marker proteins, these PBPs were estimated for their molecular weight as 106, 83, 75, 72, 63, 46, 40, and 32 kDa. Affinity of cephalexin, cefoxitin, and cefotaxime to PBPs was measured indirectly by competition for subsequent binding or Bio-PCA. PBPs 2, 3, 4, and 7 were decreased or disappeared in the electrophoregram by prebinding with these β-lactams. Two PEPs, PBP 3 and PBP 4, which were predominant in vegetative and sporulating cells, respectively, showed strong affinity for cephalexin.
Created Date 2012.08.02
Modified Date 2022.11.11

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