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＜departmental bulletin paper＞
Net Assimilation Rate of Forages and Forage Intake of Ruminants as Related using a Hypothetic Equation Suggested to Growth Analysis of both Forages and Ruminants

Creator
Author PID
K001569
Creator Name
Shimojo, Masataka
下條, 雅敬
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Creator Name
Bungo, Takashi
豊後, 貴嗣
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Creator Name
Tobisa, Manabu
飛佐, 学
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Creator Name
Imura, Yoshimi
伊村, 嘉美
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Creator Name
Koga, Naoki
古賀, 直樹
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Creator Name
Tao, Shao
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
Creator Name
Yunus, Muhammad
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
Author PID
K001674
Creator Name
Nakano, Yutaka
中野, 豊
Affiliation
Affiliation Name
Kyushu University Farm
九州大学農学部附属農場
Creator Name
Goto, Ichiro
五斗, 一郎
Affiliation
Affiliation Name
University of the Air , Fukuoka Study Center
放送大学福岡学習センター
Author PID
K000452
Creator Name
Furuse, Mitsuhiro
古瀬, 充宏
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Author PID
K001529
Creator Name
Masuda, Yasuhisa
増田, 泰久
Affiliation
Affiliation Name
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University
九州大学農学部生物資源環境学科動物生産科学飼料学講座
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1999-02
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
43
Issue
3/4
First Page
365
Last Page
371
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/24282
Related DOI
九州大学農学部紀要 || 43(3/4) || p365-371
Journal of the Faculty of Agriculture, Kyushu University || 43(3/4) || p365-371
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 43(3/4) || p365-371
Journal of the Faculty of Agriculture, Kyushu University || 43(3/4) || p365-371
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 43(3/4) || p365-371
Journal of the Faculty of Agriculture, Kyushu University || 43(3/4) || p365-371
http://www.agr.kyushu-u.ac.jp/

Hide fulltext details.

pdf p365 pdf 1.62 MB 362  

Details

PISSN
0023-6152
NCID
AA00697606
Record ID
24282
Peer-Reviewed
Unrefereed
Subject Terms
Hypothetic equation
Forage
Ruminant
ISSN
0023-6152
NCID
AA00697606
Notes
To nonradioactively detect cucumber mosaic cucumovirus (CMV) RNA with high sensitivity, we tested the use of CMV RNA-specific digoxigenin (DIG)-labeled ribonucleic acid probe, which was in 1vitro transcribed, in dot-blot, northern blot and microplate hybridization. The probe could detect up to 10 fg of CMV RNA by dot-blot hybridization with a chemiluminescent detection. Northern blot analysis showed that the riboprobe hybridizes to all CMV genomic RNAs in the total RNAs extracted from the inoculated leaves of Japanese radish without nonspecific reactions against mock-inoculated samples. Furthermore, accumulation of CMV RNA in the inoculated leaves of bottle gourd could be monitored with dot-blot and microplate hybridization. Thus, we demonstrated that the hybridization methods with nonradioactive riboprobe are applicable to highly sensitive and specific detection of CMV RNA not only in purified virion but also in total RNA extracted from host plants infected with CMV.
Created Date 2012.08.02
Modified Date 2022.11.11

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