0023-6152

AA00697606

24151

査読無

0023-6152

AA00697606

Prostaglandin E₂ (PGEa) and protein kinase C (PKC) affect proliferation of T cell and its subset which lead classical B cell isotype switching to immunoglobulin (Ig) E. In order to examine a mechanism(s) underlying different levels of circulating IgE in response to dietary saturated and polyunsaturated fats, production of PGE, and activity of PKC were measured in splenocytes of ovalbumin (OVA)-sensitized rats. Safflower oil (SO) diet-fed rats, compared to coconut oil diet-fed rats, elevated serum levels of OVA-specific IgE and IgG2a and lowered the IgG1. Activity of PKC and concanavalin (Con) A-induced T-lymphoproliferation were lowered in splenocytes from SO diet-fed rats. Production of PGE, was elevated splenocytes from SO diet-fed rats; however, this difference was disappeared when the cells were cultured with Con A. SO diet-fed rats increased proportion of linoleic acid but decreased the arachidonic acid in splenocyte phospholipids. We conclude that dietary polyunsaturated fatty acid is an important modulator of PGE, production and PKC activity in splenic immune cells and it may play a role in the circulatory level of IgE.