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＜departmental bulletin paper＞
Storage Method for Blood that Provides Stability of Erythrocyte 5-Aminolevulinic Acid Dehydratase of Carp Cyprinus carpio when Used as an Indicator of Water Lead Pollution

Creator
Creator Name
Nakagawa, Hisaki
中川, 久機
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部水産学科水産化学第一講座
Creator Name
Matsuo, Renako
松尾, 礼奈子
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部水産学科水産化学第一講座
Creator Name
Sato, Tsutomu
佐藤, 務
Affiliation
Affiliation Name
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部水産学科水産化学第一講座
Author PID
K001270
Creator Name
Watanabe, Midori
渡辺, 美登里
Affiliation
Affiliation Name
The Center of Advanced Instrumental Analysis, Kyushu University
九州大学中央分析センター
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1997-03
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
41
Issue
3/4
First Page
215
Last Page
221
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/24147
Related DOI
九州大学農学部紀要 || 41(3/4) || p215-221
Journal of the Faculty of Agriculture, Kyushu University || 41(3/4) || p215-221
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 41(3/4) || p215-221
Journal of the Faculty of Agriculture, Kyushu University || 41(3/4) || p215-221
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 41(3/4) || p215-221
Journal of the Faculty of Agriculture, Kyushu University || 41(3/4) || p215-221
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
24147
Peer-Reviewed
Unrefereed
Subject Terms
5-Aminolevulinic acid dehydratase (ALA-D)
Cyprinus carpio
Water lead pollution
ISSN
0023-6152
NCID
AA00697606
Notes
5-Aminolevulinic acid dehydratase (ALA-D) in the blood of carp Cyprinus carpio has been certified for use as an indicator of water lead pollution. The present study was undertaken in order to find storage conditions for blood samples which provide stability of, and allow a standarized expression unit for ALA-D activity, since the provision of these is essential for accurately assessing water lead pollution in a field survey, when using carp ALA-D extensively as the indicator enzyme of water lead pollution. Carp blood ALA-D levels were stable for at least 4 days when the blood was stored in a refrigerator (4℃), an ice bath, or a dry-ice bath. The three expression units of ALA-D activity, that is, per ml of RBC, ml of blood, and g of Hb, were found to be related as follows: nmol PBG/ml RBC/h = 3.6 Xnmol PBG/ml blood/h = 0.256 X nmol PBG/g Hb/h
Created Date 2012.08.01
Modified Date 2022.11.11

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