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＜departmental bulletin paper＞
Sequence Analysis of Replication Origin of Plasmid pCTP4 in y-PGA-producing Bacillus Strain isolated from “Dan-douchi” in China

Creator
Creator Name
Iwamoto, Nobuhide
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Creator Name
Nakajima, Kazuhiko
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Creator Name
Saito, Hiroyuki
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Creator Name
Kaneko, Shinji
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Creator Name
Orima, Masahito
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Author PID
K001564
Creator Name
Hara, Toshio
原, 敏夫
Affiliation
Affiliation Name
Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
九州大学農学部附属遺伝子資源研究センター微生物遺伝子部門
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1995-03
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
39
Issue
3/4
First Page
139
Last Page
148
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/24076
Related DOI
九州大学農学部紀要 || 39(3/4) || p139-148
Journal of the Faculty of Agriculture, Kyushu University || 39(3/4) || p139-148
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 39(3/4) || p139-148
Journal of the Faculty of Agriculture, Kyushu University || 39(3/4) || p139-148
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 39(3/4) || p139-148
Journal of the Faculty of Agriculture, Kyushu University || 39(3/4) || p139-148
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
24076
Peer-Reviewed
Unrefereed
Subject Terms
China
y-PGA-producing Bacillus Strain
Plasmid pCTP4
ISSN
0023-6152
NCID
AA00697606
Notes
The structure of a 1.6-kb Sphl-Hind111 DNA sequence necessary and sufficient for the replication of a 6.3-kb plasmid pCTP4 in y-PGA-producing Bacillus strain isolated from “dan-douchi” in China, which is responsible for y-polyglutamate production, has been characterized by using a trimethoprim-resistance gene derived form B. subtilis TTK24 chromosomal DNA as a selective marker. The 1.6-kb DNA sequence contains a rep gene encoding the protein (333 amino acids) essential for initiation of replication and a possible origin of replication. The predicted REP protein of pCTP4 has an overall homology with the REP proteins of pUH1 (73.9% identity), pLSl1 (87.4x), pBAA1 (82.0x), and pFTB14 (76.6%) in Bacillus spp., pLP1 (46.5%) and pLAB1000 (32.4%) in Lactobacillus spp., and pUB110 (34.8%) and pC194 (21.9%) in Staphylococcus aureus, but has not any similarity with the REP protein of the staphylococcal plasmid pT181.
Created Date 2012.08.01
Modified Date 2022.11.11

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