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＜departmental bulletin paper＞
Effect of Antigen Dose on Induction of β-Lactoglobulin-specific IgG and IgE in Brown Norway Rats

Creator
Author PID
K000847
Creator Name
Yamada, Koji
山田, 耕路
Affiliation
Affiliation Name
Laboratory of Food Science, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科食糧化学講座
Creator Name
Noda, Satoshi
Affiliation
Affiliation Name
Laboratory of Food Science, Faculty of Agriculture, Kyushu University
Creator Name
Itoh, Yuji
Affiliation
Affiliation Name
Laboratory of Food Science, Faculty of Agriculture, Kyushu University
Author PID
K000819
Creator Name
Imaizumi, Katsumi
今泉, 勝己
Affiliation
Affiliation Name
Laboratory of Nutrition Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科栄養化学講座
Author PID
L000800
Creator Name
Sugano, Michihiro
菅野, 道廣
Affiliation
Affiliation Name
Laboratory of Food Science, Faculty of Agriculture, Kyushu University
九州大学農学部食糧化学工学科食糧化学講座
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1994-12
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
39
Issue
1/2
First Page
35
Last Page
42
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/24065
Related DOI
九州大学農学部紀要 || 39(1/2) || p35-42
Journal of the Faculty of Agriculture, Kyushu University || 39(1/2) || p35-42
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 39(1/2) || p35-42
Journal of the Faculty of Agriculture, Kyushu University || 39(1/2) || p35-42
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 39(1/2) || p35-42
Journal of the Faculty of Agriculture, Kyushu University || 39(1/2) || p35-42
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
24065
Peer-Reviewed
Unrefereed
Subject Terms
Antigen
IgG
IgE
Enzyme-linked immunosorbent assay (ELISA)
ISSN
0023-6152
NCID
AA00697606
Notes
We established enzyme-linked immunosorbent assay (ELISA) which can detect 0.4-4 ng/ml of IgE by an avidin-biotin method, and 2-20 ng/ml of IgG or 5-50 ng/ml of IgM by direct ELISA methods. The ELISA methods were applied to measure serum immunoglobulin titers of Brown Norway rats intraperitoneally immunized with various amounts of fl-lactoglobulin using an alum adjuvant. Induction of antigen-specific IgG was predominant at high antigen doses such as 100 and 1000 pg/rat, but markedly decreased at low antigen doses such as 1 and 10 ,ug/rat. Induction of antigen-specific IgE was not so affected by antigen dose, but was much weaker than IgG induction and was temporal.
Created Date 2012.08.01
Modified Date 2022.11.11

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