Application of the Direct Colony TLC for Identification of Phytopathogenic Bacteria (II) Chromatographic Profile of Erwinia and Pseudomonas spp. - Collections

＜departmental bulletin paper＞
Application of the Direct Colony TLC for Identification of Phytopathogenic Bacteria (II) Chromatographic Profile of Erwinia and Pseudomonas spp.

Creator	Creator Name Matsuyama, Nobuaki 松山, 宣明 Affiliation Affiliation Name Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University 九州大学農学部農学科植物病理学講座
Creator	Author PID K001586 Creator Name Furuya, Naruto 古屋, 成人 Affiliation Affiliation Name Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University 九州大学農学部農学科植物病理学講座
Language	English
Publisher	Kyushu University
Publisher	九州大学農学部
Date	1993-12
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	38
Issue	1/2
First Page	89
Last Page	95
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/24041
Related DOI	九州大学農学部紀要 \|\| 38(1/2) \|\| p89-95
	Journal of the Faculty of Agriculture, Kyushu University \|\| 38(1/2) \|\| p89-95
	http://www.agr.kyushu-u.ac.jp/
Related URI	九州大学農学部紀要 \|\| 38(1/2) \|\| p89-95
	Journal of the Faculty of Agriculture, Kyushu University \|\| 38(1/2) \|\| p89-95
	http://www.agr.kyushu-u.ac.jp/
Relation	九州大学農学部紀要 \|\| 38(1/2) \|\| p89-95
	Journal of the Faculty of Agriculture, Kyushu University \|\| 38(1/2) \|\| p89-95
	http://www.agr.kyushu-u.ac.jp/

File	FileType	Size	Views	Description
p089	pdf	943 KB	298

PISSN	0023-6152
NCID	AA00697606
Record ID	24041
Peer-Reviewed	Unrefereed
Subject Terms	Phytopathogenic bacteria
	Thin-layer chromatography
	Erwinia
ISSN	0023-6152
NCID	AA00697606
Notes	A rapid identification of phytopathogenic bacteria was available at species level by the direct colony thin-layer chromatography. Distinct difference was observed in chromatographic profile between Emoirza’a chrysanthemi and E. carotovora subsp. carotovora. In the case of Erwinia carotovora subsp. carotovora, a characteristic spot at Rf 0.57 appeared concomitantly with a common spot at 0.62. The chromatograms of Pseudomonas spp. were divided into three major types. The chromatograms of P. gladioli pv. gladioli, P. glumae, P. plantarii, P. caryophylli and P. cepacia resembled each other. This chromatographic profile was clearly different from those of other pseudomonads and designated as a Cepacia-type. In the case of P. solanacearum, a characteristic spot appeared at Rf 0.54 and this profile was named as a Solanacearum-type. The profile of remaining pseudomonads was simple and similar each other, and was designated as a Syringae-type. A practical usefulness of this easy method was certified also in this experiment.
Created Date	2012.08.01
Modified Date	2022.11.11