As many as 92 different samples belonging to 15 botanical families, showing virus disease-like symptom were collected from various locations of Bangladesh in 1986-87. Plant samples were lyophilized or dried lvith calcium chloride and preserved at 4℃. Since inactivation of most of the samples was observed in mechanical inoculation to original or closely related host plants in 1989, the dried samples were subjected to double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and dot-immunobinding assay (DIBA) for detecting cucumber mosaic virus (CMV). Two, three, two and one of the samples of cucumber, chilli, pepper and tomato, respectively, were found to be positive against the antiserum of CMV in DAS-ELISA. Of these eight samples, six reacted positively in DIBA. Simplified double-diffusion (SDD) test was also applied to detect CMV from dried samples. All these three methods DAS-ELISA, DIBA and SDD test were found to be useful for the detection of CMV from dried samples. Results demonstrated that the antigenicity of CMV retained at least for 2-3 years after drying even if they had completely lost their infectivity. The results also suggested the occurrence of CMV on cucurbitaceous and solanaceous plants in Bangladesh, but its distribution frequency seemed not so high.